Weld Hill Microscopy Lab

Microscopists at Weld Hill will find a range of tools from the simple hand lens to the confocal microscope for 3-D reconstructions. For registered users of the facilities, login to Online Reservations to reserve the equipment.

Download microscope specs

JEOL JSM-6010 LV Scanning Electron Microscope

Resolution: 4.0 nm in HV mode; 5.0 nm in LV mode
Magnification: 80X to 300kX
Accelerating Voltage: 500 V to 20 kV
Detectors: Everhart Thornley, Backscatter with COMPO, TOPO, and Shadow, Low Vacuum Secondary Electron detector
Operating Pressure: 10-4 Pa order to 100Pa
Other: motorized 2 axis stage, chamberscope, stage navigation system

Zeiss LSM700 Confocal Microscope

Objectives: 10x/0.45 M27 Plan-Apochromat;20x/0.8 M27 Plan-Apochromat; 20x/0.5 WD 2.6 M27 W N-Achroplan; 40x/0.95 Korr M27 Plan-Apochromat; 40x/1.3 Oil Plan-Neofluar; 63x/1.40 Oil DIC M27 Plan-Apochromat; 100x/1.46 Oil DIC M27 alpha-Plan-Apochromat
Confocal Software: Zen Black 2.3
Confocal Laser Lines: 405nm, 488nm, 555nm, 639nm
Brightfield/Fluorescent Imaging Camera: AxioCam 512
Brightfield/Fluorescent Software: Zen Blue 2.3
Fluorescent Light Source: Lumencor SOLA-365-SE light engine
Fluorescent Filters:
Position 1: DAPI narrow (Chroma Custom set) excitation G 365/10; dichroic mirror T 385 LP; emission 400 LP;
Position 2: DAPI wide (Zeiss Filter set 49, 488049-0000) excitation G 365; beamsplitter FT 395; emission BP 445/50;
Position 3: FITC/AF488/GFP minus chlorophyll auto-fluorescence (Zeiss Filter set 20, 488020-0000) excitation BP 546/12; beamsplitter FT 560; emission BP 575-640;
Position 4: Rhodamine (Zeiss Filter set 20, 488020-0000) excitation BP 546/12; beamsplitter FT 560; emission BP 575-640

Zeiss Discovery v12 Dissecting microscope

Objectives: 0.63x PlanApo S; 1.5x PlanApo S; 2.3x PlanApo S
Camera: AxioCam 512
Software: Zen Blue Pro with extended focus and time lapse modules

Zeiss AxioImager A2 Upright microscope

Objectives: 2.5x/0.075 EC Plan-Neofluar; 5x/0.16 EC Plan-Neofluar; 10x/0.45 Plan-Apochromat; 20x/0.8 Plan-Apochromat; 40x/0.95 Korr Plan-Apochromat; 63x/1.40 Plan-Apochromat Oil DIC
Camera: AxioCam 512
Software: Zen Blue Pro with multichannel
Fluorescent Light Source: Lumencor SOLA-365-SE light engine
Fluorescent Filters:
Position 1: DAPI narrow (Chroma Custom set) excitation G 365/10; dichroic mirror T 385 LP; emission 400 LP;
Position 2: FITC/AF488/GFP plus chlorophyll auto-fluorescence (Chroma 49012) excitation: BP 480/40; beamsplitter: FT 510; emission: 510 LP
Position 3: FITC/AF488/GFP minus chlorophyll auto-fluorescence (Zeiss Filter set 38, 1031-346) excitation BP 470/40; beamsplitter FT 495; emission BP 525/50;
Position 4: Rhodamine (Zeiss Filter set 20, 488020-0000) excitation BP 546/12; beamsplitter FT 560; emission BP 575-640

Zeiss Axiophot

Microscope: Zeiss Axiophot
Objectives: 5x/0.15 Plan-Neofluar; 10x/0.30 Plan-Neofluar Pol; 20x/0.5 Plan-Neofluar Pol; 40x/0.85 Plan-Neofluar Pol; 63x/1.4 Plan-Apochromat Oil DIC
Camera: AxioCam 512
Software: Zen Blue with multichannel
Fluorescent Light Source: Lumencor SOLA-365-SE light engine
Fluorescent Filters:
Position I: DAPI narrow (Zeiss 48702) excitation G 365, bandpass 12 nm; dichroic mirror FT 395; barrier filter LP397;
Position II: DAPI wide (Chroma 49000) excitation G 350/50; dichroic mirror FT 400; barrier filter 460/50;
Position III: AF488/FITC/GFP (Zeiss Filter set 9) excitation 450-490; dichroic mirror 510; emission filter LP515;
Position IV: Rhodamine (Zeiss Filter set 15) excitation 540-560 nm; dichroic mirror FT 580; barrier filter LP590

Zeiss Axioskop

Microscope: Zeiss Axioskop
Objectives: 2.5x/0.075 Plan-Neofluar; 10x/0.30 Plan-Neofluar Ph1; 20x/0.5 Plan-Neofluar Ph2; 40x/0.75 Plan-Neofluar Ph2
Camera: AxioCam HRc
Software: Zen Blue with multichannel
Fluorescent Light Source: Lumencor SOLA-365-SM light engine (Before March 2015, HBO 50-W)
Fluorescent Filters:
Position I: DAPI narrow (Zeiss 48702) excitation G 365, bandpass 12 nm; dichroic mirror FT 395; barrier filter LP397;
Position II: Rhodamine (Zeiss Filter set 15) excitation 540-560 nm; dichroic mirror FT 580; barrier filter LP590;
Position III: AF488/FITC/GFP (Zeiss Filter set 9) excitation 450-490; dichroic mirror 510; emission filter LP515

Zeiss Microspectrophotometer

Microspectrophotometer: Zeiss MSP 20
Microscope: Zeiss Axioskop
Objectives: 10x/0.30 Plan-Neofluar; 20x/0.5 Plan-Neofluar; 40x/0.75 Plan-Neofluar Pol; 100x/1.3 Plan-Neofluar Oil; 100x/1.3 Plan-Neofluar Oil Iris
Fluorescent Light Source: HBO 100-W burner
Fluorescent Filters: DAPI (Zeiss Filter set 48702) excitation G 365, bandpass 12 nm; dichroic mirror FT 395; barrier filter LP397