Microscopy Lab

Weld Hill Microscopy Lab

Microscopists at Weld Hill will find a range of tools from the simple hand lens to the confocal microscope for 3-D reconstructions. For registered users of the facilities, login to Online Reservations to reserve the equipment.

Download microscope specs

JEOL JSM-6010 LV Scanning Electron Microscope

Resolution: 4.0 nm in HV mode; 5.0 nm in LV mode
Magnification: 80X to 300kX
Accelerating Voltage: 500 V to 20 kV
Detectors: Everhart Thornley, Backscatter with COMPO, TOPO, and Shadow, Low Vacuum Secondary Electron detector
Operating Pressure: 10-4 Pa order to 100Pa
Other: motorized 2 axis stage, chamberscope, stage navigation system

Zeiss LSM700 Confocal Microscope

Objectives: 10x/0.45 M27 Plan-Apochromat;20x/0.8 M27 Plan-Apochromat; 20x/0.5 WD 2.6 M27 W N-Achroplan; 40x/0.95 Korr M27 Plan-Apochromat; 40x/1.3 Oil Plan-Neofluar; 63x/1.40 Oil DIC M27 Plan-Apochromat; 100x/1.46 Oil DIC M27 alpha-Plan-Apochromat
Confocal Software: Zen Black 2.3
Confocal Laser Lines: 405nm, 488nm, 555nm, 639nm
Brightfield/Fluorescent Imaging Camera: AxioCam 512
Brightfield/Fluorescent Software: Zen Blue 2.3
Fluorescent Light Source: Lumencor SOLA-365-SE light engine
Fluorescent Filters:
Position 1: DAPI narrow (Chroma Custom set) excitation G 365/10; dichroic mirror T 385 LP; emission 400 LP;
Position 2: DAPI wide (Zeiss Filter set 49, 488049-0000) excitation G 365; beamsplitter FT 395; emission BP 445/50;
Position 3: FITC/AF488/GFP minus chlorophyll auto-fluorescence (Zeiss Filter set 20, 488020-0000) excitation BP 546/12; beamsplitter FT 560; emission BP 575-640;
Position 4: Rhodamine (Zeiss Filter set 20, 488020-0000) excitation BP 546/12; beamsplitter FT 560; emission BP 575-640

Zeiss Discovery v12 Dissecting microscope

Objectives: 0.63x PlanApo S; 1.5x PlanApo S; 2.3x PlanApo S
Camera: AxioCam 512
Software: Zen Blue Pro with extended focus and time lapse modules

Zeiss AxioImager A2 Upright microscope

Objectives: 2.5x/0.075 EC Plan-Neofluar; 5x/0.16 EC Plan-Neofluar; 10x/0.45 Plan-Apochromat; 20x/0.8 Plan-Apochromat; 40x/0.95 Korr Plan-Apochromat; 63x/1.40 Plan-Apochromat Oil DIC
Camera: AxioCam 512
Software: Zen Blue Pro with multichannel
Fluorescent Light Source: Lumencor SOLA-365-SE light engine
Fluorescent Filters:
Position 1: DAPI narrow (Chroma Custom set) excitation G 365/10; dichroic mirror T 385 LP; emission 400 LP;
Position 2: FITC/AF488/GFP plus chlorophyll auto-fluorescence (Chroma 49012) excitation: BP 480/40; beamsplitter: FT 510; emission: 510 LP
Position 3: FITC/AF488/GFP minus chlorophyll auto-fluorescence (Zeiss Filter set 38, 1031-346) excitation BP 470/40; beamsplitter FT 495; emission BP 525/50;
Position 4: Rhodamine (Zeiss Filter set 20, 488020-0000) excitation BP 546/12; beamsplitter FT 560; emission BP 575-640

Zeiss Axiophot

Microscope: Zeiss Axiophot
Objectives: 5x/0.15 Plan-Neofluar; 10x/0.30 Plan-Neofluar Pol; 20x/0.5 Plan-Neofluar Pol; 40x/0.85 Plan-Neofluar Pol; 63x/1.4 Plan-Apochromat Oil DIC
Camera: AxioCam 512
Software: Zen Blue with multichannel
Fluorescent Light Source: Lumencor SOLA-365-SE light engine
Fluorescent Filters:
Position I: DAPI narrow (Zeiss 48702) excitation G 365, bandpass 12 nm; dichroic mirror FT 395; barrier filter LP397;
Position II: DAPI wide (Chroma 49000) excitation G 350/50; dichroic mirror FT 400; barrier filter 460/50;
Position III: AF488/FITC/GFP (Zeiss Filter set 9) excitation 450-490; dichroic mirror 510; emission filter LP515;
Position IV: Rhodamine (Zeiss Filter set 15) excitation  540-560 nm; dichroic mirror FT 580; barrier filter LP590

Zeiss Axioskop

Microscope: Zeiss Axioskop
Objectives: 2.5x/0.075 Plan-Neofluar; 10x/0.30 Plan-Neofluar Ph1; 20x/0.5 Plan-Neofluar Ph2; 40x/0.75 Plan-Neofluar Ph2
Camera: AxioCam HRc
Software: Zen Blue with multichannel
Fluorescent Light Source: Lumencor SOLA-365-SM light engine (Before March 2015, HBO 50-W)
Fluorescent Filters:
Position I: DAPI narrow (Zeiss 48702) excitation G 365, bandpass 12 nm; dichroic mirror FT 395; barrier filter LP397;
Position II: Rhodamine (Zeiss Filter set 15) excitation  540-560 nm; dichroic mirror FT 580; barrier filter LP590;
Position III: AF488/FITC/GFP (Zeiss Filter set 9) excitation 450-490; dichroic mirror 510; emission filter LP515

Zeiss Microspectrophotometer

Microspectrophotometer: Zeiss MSP 20
Microscope: Zeiss Axioskop
Objectives: 10x/0.30 Plan-Neofluar; 20x/0.5 Plan-Neofluar; 40x/0.75 Plan-Neofluar Pol; 100x/1.3 Plan-Neofluar Oil; 100x/1.3 Plan-Neofluar Oil Iris
Fluorescent Light Source: HBO 100-W burner
Fluorescent Filters: DAPI (Zeiss Filter set 48702) excitation G 365, bandpass 12 nm; dichroic mirror FT 395; barrier filter LP397

Zeiss Stemi SV 11

Objectives: 1.0x Plan Apochromat S; 1.6x Plan S
Camera: AxioCam HRc
Software: Zen Blue

Zeiss Stemi 2000-C

Objectives: 1.0x Plan Apochromat S; 1.6x Plan S

critical point dryerTousimis Autosamdri-815 Series A

Critical Point Dryer

Vacuum evaporatorDenton Vacuum Desk II

Sputter Coater

BP-111-RS Lab Microwave

Care of microwave

Microwave fixation for RNA; Microwave processing and immunolocalization

microwave mounting of slides

TBS Embedding Station

Care of embedding center

Embedding

Paraffin OvenMemmert Paraffin Oven

UN30pa

Thermo Scientific HM360 Microtome (Resin)

Leica RM2155 Microtome (Resin)

Leica_RM2155 Manual

Microtome (Paraffin)

Sectioning 101

Reichert-Jung 2800 Frigocut E (Frozen)

RMC MT-7 Ultra Microtome (Ultra thin)

VibratomeTPI Vibratome 1000/2000 (Fresh)

More Protocols

Feulgen staining